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"Post-Workout Testosterone": New Research and why “Bro Science" carries more weight than it should

I feel a bit vindicated in one of my opinions. That’s because for years I’ve been asserting that ‘post-workout testosterone’ doesn’t really matter very much for bodybuilding. This has flown counter to what’s repeated, ad nauseum, by the bodybuilding community. They’ve been warning natural trainees within every book, report, and magazine article about the hormonal shortfalls and resulting muscle gain plateaus that will surely befall anyone who takes bodybuilding workouts past the one-hour time mark:

“Don’t stay in the gym more than one hour”, they’ll say. “Your testosterone will begin to drop and that’s not what you want for bodybuilding progress.”

But just a little common sense rejects this notion. The same geniuses who’ve been dispensing this advice have been simultaneously meting out information that’s not completely reconcilable with it: That “muscles don’t grow during workouts; they grow during rest between workouts.”

So again I ask: “Why should workout testosterone levels, or even ‘post-workout testosterone’ levels, matter if muscles are only growing during rest time in the days between workouts?”


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The ‘post-workout testosterone’ crowd might counter this argument by claiming there’s a “window of opportunity” immediately following a workout in which we want endogenous testosterone to be high. During this window, the body gets a jump on recuperation by using post-workout testosterone and other raised hormone levels to kick-start protein synthesis. This has been so oft repeated that fitness gurus have apparently taken to merely saying it because they hear every other “expert” say it.

Now there’s evidence that casts serious doubt on the idea that ‘post workout testosterone’ is something of which to be concerned. The first of such evidence comes in the form of a study done on sex-based comparisons of post-workout myofibrillar protein synthesis (MPS). The study was done on eight men and eight women at McMaster University’s Kinesiology Department in Ontario Canada.  The most surprising finding of this study was that post-workout stimulation of MPS was as pronounced in the women as it was in the men. This is significant given that women possess about 5 to 10 percent of the endogenous testosterone that men do.

‘Post Workout Testosterone’: Evidence that it matters

The idea that in-workout and ‘post workout testosterone’ are pivotal factors for muscle building progress isn’t without supporting evidence. A 2011 study done at the Norwegian School of Sports Sciences at Lillehammer University in Oslo, Norway showed that acute hormone boosts from training make a difference to strength gains. Researchers took twelve untrained male subjects and had them perform a four-workouts-per-week unilateral strength-training protocol for 11 weeks. The subjects each trained the arm muscles of one side of the body twice-per-week with a heavy leg training session preceding the arms-training bouts. They trained the arm muscles of the other sides of their bodies for two training sessions per week without the accompanying leg training. The respective side of each subject’s body that received arm workouts without the leg workouts was used as a ‘control group.’ By doing the study unilaterally like this, the researchers could ensure that the same nutritional variables were in play for both the experimental and control sides of the study. Acute hormonal responses and corresponding strength gains from the arm-training workouts were monitored and measured.

Loading WeightsWhat were the results of the study?

Serum growth hormone and testosterone levels increased significantly immediately following the training sessions in which legs were trained along with arms. This was in contrast to no significant hormonal changes occurring from the arms-only workouts. There was no substantial change in serum cortisol levels from either of the two types of training sessions.

The difference in arms strength gains between the experimental and control groups was noteworthy. There was a 21% increase in one-repetition-max biceps-curling strength of the arms/legs workout group. This is in contrast to a 14% increase in biceps-curling strength for the ‘arms only’ group. In addition, the arms/legs group had a greater increase in CSA (cross-sectional area) mass in the middle sections of the elbow flexors where the CSA of the flexors is largest.

The researchers also measured ‘peak power’ of biceps strength during the study. This was done by testing the biceps strength at both 30% and 60% of 1RM, respectively. Using this measurement, there were no statistical differences between the arms/legs training groups and the ‘arms only’ groups.

This study showed that the transient nature of ‘post workout testosterone’ and other acute hormone augmentation could make a difference to strength adaptation.

‘Post Workout Testosterone’: Evidence that it doesn’t matter

There’s recently been a rather thorough research study, however, resulting in evidence that ‘post workout testosterone’ spikes amount to not much more than an interesting blood-readout phenomena of a very transient nature.  And it’s the transient nature of this spike that has to cause any thinking person a reason for pause. Think about it: Steroid users accelerate their muscle/strength gains by raising their testosterone levels six-fold above normal with either oral or intravenous use of those drugs. Not only is this amount of testosterone way above normal – it’s also jacking up the drug-users level of this hormone on a 24/7 basis while he or she is on the steroid cycle. Although the user’s subsequent bigger gains are direct evidence that higher testosterone levels can result in significant contractile tissue accretion – it might likewise be indirect evidence that such accretion can only be attained with levels and consistencies acquired by pharmacological means.

The study was conducted to test the effects of the natural testosterone spike. Researchers wondered if the post workout testosterone rise, along with upswings in other post-workout hormones, results in better adaptations to resistance training. They conducted it on 56 young men (a huge cohort) in order to obtain more accuracy than similar studies in the past. The subjects underwent a 12-week workout protocol of training 5 days per week on intense weight lifting with both upper and lower body exercises. Their lean body mass was measured. Their blood readings of testosterone, HGH, IGF-1, and cortisol were measured within the 120-minute post workout time window. Muscle biopsies were taken on the cross-sectional area of their vastus lateralus (thigh muscle) both before and at the finish of the study. The subject’s leg press strength was tested before and after the experiment. And of course, firm statistical analysis was applied to the compilation of all these findings.

What were the results of the study?

In a nutshell, ‘post workout testosterone’ spikes and other acute hormonal responses showed no significant association with gains in lean body mass or increases in leg pressing strength. Some of the subjects gained significant strength and mass, while some gained almost nothing, and many gained something of a mediocre amount. But what might be telling is the fact that the top 16% of gainers in strength and lean body mass showed no greater hormonal output than the bottom 16% of strength and muscle gain responders.

Interestingly, the researchers found a weak correlation between elevation of cortisol (along with HGH) and increases in CSA of Type 2 muscle fibers. This was especially counterintuitive considering that cortisol is known to be a catabolic hormone and HGH has collectively been shown in past studies to be more gluconeogenic and metabolism-modulating than anabolism-enhancing.

Post Workout Testosterone’ Studies… and why “bro science” carries weight

In light of the above-mentioned studies, there currently appears to be two opposing dispensers of information in the bodybuilding world. There are those who only practice bodybuilding and share their street-gained knowledge of it with others. In occasional antagonism to this, there are individuals within the vanguard of bodybuilding and strength training’s University research groups who label the former as propagators of mere “bro science” – as in “science” of such utter ‘junk’ variety that it originates and proliferates with about as much objectivity as is implied by the following revelatory sentence:

“Hey Bro, why don’t you try THIS to gain muscle… it worked for me.”

Understandably, there’s apparent disdain within the research community for the ongoing dissemination of bodybuilding and fitness information in the latter form. Research teams, with PhDs in biology, kinesiology and nutrition, etc., have legitimate reason to convey the impact and importance of their controlled research work above anecdotally-derived, individual discoveries that can mistakenly thought to be all-encompassing and conclusive.

What might be noted, however, is the present relative inconclusiveness from even the ‘scientific’ findings within bodybuilding and strength training. Take the above-cited studies on ‘post workout testosterone’ as example. Even the researchers of the third study – the most thorough of the three mentioned – admit in their summary that more research is needed before a complete understanding of exercise-induced, acute hormone elevations can be fully understood.

Where does that leave serious natural bodybuilders until the ‘science’ of this industry further unfolds?

It likely leaves them more legitimately relying on “bro science” by omission of un-contradictory scholarly knowledge than researchers within the industry care to realize.


1. Daniel W. D. West, Nicholas A. Burd, Tyler A. Churchward-Venne, Donny M. Camera, Cameron J. Mitchell, Steven K. Baker, John A. Hawley, Vernon G. Coffey, and Stuart M. Phillips.  ‘Sex-based comparisons of myofibrillar protein synthesis after resistance exercise in the fed state’ (Journal of Applied Physiology, June 1, 2012, vol. 112, no. 11 1805-1813)
2. Ronnestad, B.R., Nygaard, H., Raastad, T. (2011) ‘Physiological elevation of endogenous hormones results in superior strength training adaptations.’ (European Journal of Applied Physiology, 111, 2249-2259)
3. Daniel W.D. West, Stuart M. Phillips. ‘Associations of exercise-induced hormone profiles and gains in strength and hypertrophy in a large cohort after weight training’ (European Journal of Applied Physiol. 2012 July; 112(7): 2693–2702.)




I've only added it this week to be honest so not enough time to see if it is working. I eat a few chunks as a snack as liver is not easy to eat in large doses. On the calorie side of things you are absolutely right we have to watch our calorie and fat intake that is why I'm eating liver.

Lamb Liver:
Lamb liver has the highest fat and cholesterol content of all the livers, with one serving containing 5 g of fat and 371 mg of cholesterol. One serving also contains 139 calories, 20.4 g of protein and 1.8 g of carbohydrates. The 7.4 mg of iron in each serving provides more than 90 percent of the DRI for men and more than 40 percent for women. One serving of lamb liver also provides more than 100 percent of the DRI of vitamin A riboflavin, niacin, pantothenic acid and B-12, as well as more than 50 percent of B-6 and folate.

As you can see one serving 100grams/3.5oz is only 139 calories not bad for a super food. I will keep you informed on progress. Even if the liver isn't so great in helping with testosterone for what ever reasons it is still a good way to snack and get some extra protein 20.4 grams inn a serving. Also makes a change from Tuna.

Scott Abbett


Thanks for bringing that up since there are still a lot of guys who aren't aware of it. They're stuck in the past, still thinking they need to cut their fat and cholesterol intake down to 10% or less of their ratio of daily calories. Instead, they need to keep a couple of egg yokes in with that egg white omellete, and a bit of the fat on the edges of those strips of top sirloin.

But this can also be taken too far. It's still important to remember that high fat/cholesterol food is dense with calories. Too many calories causes fat gain and body fat can greatly reduce exogenous testosterone.

Years ago, when I saw somewhere that monounsaturated fat is (also) important for testosterone production, I REALLY took it to heart. I saw it as a green light to start dumping more Olive Oil into a daily bodybuilding smoothie I was consuming at the time. Low-and-behold... I began getting fatter around the waistline. The additional calories I was taking in were NOT being offset by whatever (if any) additional T-level I was gaining from the increased dietary fat. I'd conveniently adopted an implicit assumption that they would since testosterone is a fat-burning hormone and (what-the-hell) the additional Olive Oil was improving the flavor and consistency of the smoothies.

My point: Just because fat/cholesterol is required for testosterone production doesn't mean those things will necessarily stimulate more production. There's a certain amount of cholesterol that's needed, along with a low-threshold limit and a 'law of diminishing return' kicking in somewhere. If a guy's getting enough cholesterol to produce testosterone and low T-levels are due to something else, then additional cholesterol won't help and might even hurt.

Just some things to keep in mind.


P.S. Have you added the liver to your diet? If so, did you notice results?


As so happens in my research on boosting Testosterone (naturally) I've come across that demonised food/nutrient Cholesterol as it happens this stuff is so important in creating Testosterone (nut juice) it's unbelievable how much importance is given to protein but, all the protein in the world is of no use in the absence/lack of Testosterone. One food Ive found to give a double whammy is Liver high in protien and Cholesterol which in turn should help boost Testosterone especially for guys like me in there 50's.

Scott Abbett

Hey Sammy,

That sounds good - especially the part about it being effective for you. You should post some online pics depicting your progress sometime.

Thanks for your input.


Scott Abbett

Hello Dai,

Great question! If the two hours after a workout is as important as so many experts would have us believe, you'd think there'd be studies showing that optimizing everything at that time would knock days off our overall inter-workout recuperation time requirement.

You're right - the guys you've mentioned developed impressive physiques without whey protein,Carnivore protein,creatine, tribulus,Carnitine,BCAA supplements, or a post-workout "recovery drink" that digests thirty seconds after the conclusion of a workout.

Then again,Vince Gironda used to advocate adding dessicated liver tabs to bodybuilder's diets. It's funny to recall how a buddy of mine in high school used to take those by the handful.

Thank you for your comment.



Oh and the most important principle: progressive overload. I switched to shorter rests between sets, too, and have found that beneficial.


For me, it has come down to the KISS method. I've simplified, moving to a 4-day, upper/lower body split, lifting in a couple of different rep ranges (6-8 and 9-12), focusing mostly on the "big" lifts (bench, squat, deadlift, weighted pullup/barbell row, dips), with some supplemental, accessory lifts following. Early strength and bodybuilder types used these and full body workouts to get big and strong. So far, the results have been the best of my life.


Scott why does it take two maybe three days to recover if post workout everything is so important. Even more important is why did guy's like Vince Gironda, Steve Reeves do so well on different diets with little to no supplementation and definitely no pre or post workout drink food or most anything else for that matter. We have to remember that guy's from back in the 50's, 60's, and 70's didn't have the supplements or the knowledge we have today.

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